The premier DNA and drug testing company in the North Texas area
EtG Alcohol Test
Alcohol is rapidly eliminated from the body at a rate of approximately one drink per hour. Whether looking for alcohol in breath, blood, saliva or urine, using the standard technology, the rapid elimination limits the detection of alcohol to a matter of hours.
For example an individual who was “under the influence” of alcohol using standard technologies (breath, blood or saliva > 0.08%) at 10 PM would likely test negative the next morning at 9 AM due to the rapid elimination of alcohol from the body.
After years of research, Ethyl Glucuronide (EtG) was found to be a direct metabolite of the alcohol (ethanol). EtG has emerged as the marker of choice for alcohol and due to the advances in technologies is now routinely available. Its presence in urine may be used to detect recent alcohol consumption, even after ethanol is no longer measurable using the older methods. The presence of EtG in urine is a definitive indicator that alcohol was ingested. Other types of alcohol, such a stearyl, acetyl and dodecanol, metabolizes differently and will not cause a positive result on an EtG test.
The EtG test has become known as the “80 hour test” for detecting any amount of consumed ethyl alcohol. This is not totally true. It is true that EtG can be detected in chronic drinkers for 80 hours or even up to 5 days. During this period of chronic use, the EtG level can exceed 100,000 ng/mL. Two primary factors to determine the window of detection is based on volume of alcohol consumed and the time between each drink. A person that consumes 3 drinks can only have a detectable level of EtG for approximately 20 to 24 hours and peaks at approximately 9 hours with an EtG level around 15,000 ng/mL.
Therefore, the presence of EtG in urine indicates that ethanol was ingested. EtG is a more accurate indicator of recent consumption of alcohol than measuring for the presence of ethanol itself.
EtG is stable in urine for more than 4 days at room temperature. Recent experiments indicate that heating urine to 100 degrees C actually increased the stability of EtG. Therefore, heat does not cause the breakdown of EtG, it actually increases stability. In addition, no artificial formation of EtG was found to occur following the prolonged storage of urine at room temperature fortified with 1% ethanol.
EtG is a direct metabolite of alcohol (ethanol), and its detection in urine is highly specific, similar to testing for other drugs. The typical lab utilizes the most sophisticated, sensitive, and specific equipment and technology available, LC/MS/MS, to screen, confirm, and quantitative EtG. This methodology provides highly accurate results.
Studies indicate that alcoholics in abstinence have no detectable levels of EtG in their urine after approximately 80 hours of detoxification.
EtG is only detected in urine when alcohol is consumed. This is important since it is possible to have alcohol in urine without drinking. Alcohol in urine without drinking is due to the production of ethanol in vitro. Ethanol in vitro is spontaneously produced in the bladder or the specimen container itself, due to fermentation of urine samples containing sugars (diabetes) and yeast or bacteria. Since the ethanol produced is not metabolized by the liver, EtG will not be produced and will therefore not be detected in a urine containing alcohol as a result of fermentation.
Tests show that “incidental exposure” to the chronic use of food products (vanilla extract), hygiene products, mouthwash, or OTC medications (cough syrups) can produce EtG concentrations in excess of 100 ng/mL. However, if measurable ethanol is detected (greater than .04 gm%) in the urine, and EtG is also detected in excess of 250 ng/mL, then this is very strong evidence that beverage alcohol was consumed.
Labs will allow you to select 100, 250 or 500 ng/mL as the cutoff level. It is strongly recommended that only the 500 ng/mL level be used. This avoids and eliminates any claim by the donor that the positive EtG test is a result of incidental or unintentional exposure. All testing performed on products or foods classified as incidental or unintentional exposure has never produced a positive EtG level greater than 500 ng/mL.
EtG alcohol testing is being performed in hair and nails. The window of detection is approximately 3 months for head or body hair and finger or toe nails. But be aware that biase does exist when comparing male and female hair. Recently an Italian study reported that bleaching the hair completely destroys EtG. Another study with water from a commercial pool was performed to determine the effects of chlorine. Hair exposed to the pool water found that two, 20 minute exposures reduced EtG by approximately 20%. It was found that this did not have any effect to alter or reduce the drug levels in nails. Based on these facts alone, it is recommended that only nails be used to test for EtG and not hair.
DNA testing has changed over the years. It is now as simple as collecting a saliva swab from the inside of the cheek. Samples from the child and alleged father will provided you a notarized, court admissible DNA parentage test. When properly collected, the report will state that you are the father (99.99%) or not the father (0.0%).
Both court admissible & non-court admissible DNA Parentage tests available. Contact us for pricing.
Forensic DNA & Drug Testing Services, Inc. also provides other types of DNA testing. Siblings, grandparents, aunts and uncle may participate if the alleged father is deceased or unavailable due to incarceration or other reasons. All types of DNA testing are available such as criminal cases or infidelity testing for sperm, semen and saliva.
Know the intent of why you need to have a DNA test. Many DNA kits are available on the market through websites at very low prices but are NOT admissible for legal cases, social security, immigration, inheritance, etc.
Please call any of our offices to discuss specifics related to your case and for pricing of other types of testing.
LOCATIONS AND COLLECTION SITES
Two offices in the north Texas areas are conveniently located to collect samples for your legally, court admissible DNA test. If a person is located in another city, state or country, one of our third party partners will collect the swab(s) maintaining the proper collection for the results to be court admissible.